Detection of Proteins on Blot Membranes
نویسندگان
چکیده
منابع مشابه
Detection of Proteins on Blot Membranes.
Staining of blot membranes enables the visualization of bound proteins. Proteins are usually transferred to blot membranes by electroblotting, by direct spotting of protein solutions, or by contact blots. Staining allows the efficiency of transfer to the membrane to be monitored. This unit describes protocols for staining proteins after electroblotting from polyacrylamide gels to blot membranes...
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Immunodetection of proteins on blot membranes, Wrst developed by Towbin et al. [1], is the method of choice for identifying speciWc proteins after electrophoresis. Due to its high resolution, immunoprobing of blots from twodimensional polyacrylamide gels has been used extensively as a means of localizing speciWc proteins among hundred or even thousands of separated proteins [2]. Three major typ...
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ژورنال
عنوان ژورنال: Current Protocols in Protein Science
سال: 2016
ISSN: 1934-3655,1934-3663
DOI: 10.1002/cpps.15